La plateforme MetaToul peut être sollicitée par la communauté scientifique publique et privée pour des analyses qualitatives et quantitatives de métabolites végétaux et microbiens pour :

• Réaliser des prestations de service pour répondre à des besoins ponctuels
• Développer de nouvelles méthodes. Une collaboration ou un partenariat peut être élaboré dans le cadre du montage et de la mise en œuvre de projets de recherche.
• Mettre à disposition des instruments analytiques.

Activités de Recherche et Développement

Métabolomique Globale :

Déréplication d’un extrait complexe

Analyses multivariées (classification, régressions, analyses discriminantes)

 Approches multi-omiques

 Métabolomique Ciblée : 

Métabolites végétaux : phytoalexines, d’alcaloïdes, composés phénoliques, flavonoïdes, …

Métabolites microbiens : lipochitooligosaccharides (LCOs), chitooligosaccharides (COS) …

Une collaboration ou un partenariat peuvent être élaborés dans le cadre de la mise en œuvre de projets de recherche.

Publications

Pedro G Vásquez-Ocmín, Guillaume Marti, Alice Gadea, Guillaume Cabanac, Juan A Vásquez-Briones, Sandro Casavilca-Zambrano, Nadia Ponts, Patricia Jargeat, Mohamed Haddad, Stéphane Bertani. 2023. ‘Metabotyping of Andean pseudocereals and characterization of emerging mycotoxins’. https://doi.org/10.1016/j.foodchem.2022.135134

Bram Danneels, Monique Blignaut, Guillaume Marti, Simon Sieber, Peter Vandamme, Marion Meyer, Aurélien Carlier. 2023. ‘Cyclitol metabolism is a central feature of Burkholderia leaf symbionts’. https://doi.org/10.1111/1462-2920.16292

Ximena Chirinos, Shiyu Ying, Maria Aurineide Rodrigues, Elie Maza, Anis Djari, Guojian Hu, Mingchun Liu, Eduardo Purgatto, Sylvie Fournier, Farid Regad, Mondher Bouzayen, Julien Pirrello. 2023. ‘Transition to ripening in tomato requires hormone-controlled genetic reprogramming initiated in gel tissue’. https://doi.org/10.1093/plphys/kiac464

Ana, Romeo-Oliván, Justine Chervin, Coralie Breton, Thierry Lagravère, Jean Daydé, Bernard Dumas, Alban Jacques. 2022. ‘Comparative Transcriptomics Suggests Early Modifications by Vintec® in Grapevine Trunk of Hormonal Signaling and Secondary Metabolism Biosynthesis in Response to Phaeomoniella chlamydospora and Phaeoacremonium minimum’. https://doi.org/10.3389/fmicb.2022.898356

Houssein, Zhour, Fabrice Bray , Israa Dandache, Guillaume Marti, Stéphanie Flament, Amélie Perez, Maëlle Lis, Llorenç Cabrera-Bosquet, Thibaut Perez, Cécile Fizames, Ezekiel Baudoin, Ikram Madani, Loubna El Zein, Anne Aliénor Véry, Christian Rolando, Hervé Sentenac, Ali Chokr, Jean-Benoît Peltier. 2022. ‘Wild Wheat Rhizosphere-Associated Plant Growth-Promoting Bacteria Exudates: Effect on Root Development in Modern Wheat and Composition’. https://doi.org/10.3390/ijms232315248

Fabio, Espichán , Rosario Rojas , Fredy Quispe, Guillaume Cabanac, Guillaume Marti. 2022. ‘Metabolomic characterization of 5 native Peruvian chili peppers (Capsicum spp.) as a tool for species discrimination’. https://doi.org/10.1016/j.foodchem.2022.132704

Chervin, Justine, Ana Romeo-Oliván, Sylvie Fournier, Virginie Puech-Pages, Bernard Dumas, Alban Jacques, and Guillaume Marti. 2022. ‘Modification of Early Response of Vitis Vinifera to Pathogens Relating to Esca Disease and Biocontrol Agent Vintec® Revealed By Untargeted Metabolomics on Woody Tissues’. Frontiers in Microbiology 13. https://www.frontiersin.org/article/10.3389/fmicb.2022.835463.

Vásquez-Ocmín, Pedro G., Guillaume Marti, Maxime Bonhomme, Fabienne Mathis, Sylvie Fournier, Stéphane Bertani, and Alexandre Maciuk. ‘Cannabinoids vs. Whole Metabolome: Relevance of Cannabinomics in Analyzing Cannabis Varieties’, 2021. https://doi.org/10.1101/2021.06.07.447363.

Vásquez-Ocmín, Pedro G., Alice Gadea, Sandrine Cojean, Guillaume Marti, Sébastien Pomel, Anne-Cécile Van Baelen, Liliana Ruiz-Vásquez, et al. « Metabolomic Approach of the Antiprotozoal Activity of Medicinal Piper Species Used in Peruvian Amazon ». Journal of Ethnopharmacology 264, 2021, 113262. https://doi.org/10.1016/j.jep.2020.113262.

Vial, Thomas, Guillaume Marti, Dorothée Missé, et Julien Pompon. « Lipid Interactions Between Flaviviruses and Mosquito Vectors ». Frontiers in Physiology 12, 2021, 763195. https://doi.org/10.3389/fphys.2021.763195.

M. Khatib, C. Pouzet, C. Lafitte, J. Chervin, V. Bonzon-Ponnet, A. Jauneau, M.-T. Esquerré-Tugayé, Phenolic profile of a Parma violet unveiled by chemical and fluorescence imaging, AoB PLANTS, 2021;, plab041, https://doi.org/10.1093/aobpla/plab041

Fraisier-Vannier, O.; Chervin, J.; Cabanac, G.; Puech, V.; Fournier, S.; Durand, V.; Amiel, A.; André, O.; Benamar, O. A.; Dumas, B.; Tsugawa, H.; Marti, G. MS-CleanR: A Feature-Filtering Workflow for Untargeted LC–MS Based Metabolomics. Anal. Chem. 2020, acs.analchem.0c01594. https://doi.org/10.1021/acs.analchem.0c01594.

Vial, T.; Tan, W.-L.; Deharo, E.; Missé, D.; Marti, G.; Pompon, J. Mosquito Metabolomics Reveal That Dengue Virus Replication Requires Phospholipid Reconfiguration via the Remodeling Cycle. Proc Natl Acad Sci USA 2020, 202015095. https://doi.org/10.1073/pnas.2015095117.

Pons, S., Fournier, S., Chervin, C., Becard, G., Rochange, S., Frei-dit-frey, N., and Puech-pages, V. Phytohormone production by the arbuscular mycorrhizal fungus Rhizophagus irregularis. PLoS ONE, 15(10):e0240886. 2020. https://doi.org/10.1371/journal.pone.0240886.

Rush, T.A., Puech-Pagès, V., Bascaules, A., Jargeat, P., Maillet, F., Haouy, A., et al. Lipo-chitooligosaccharides as regulatory signals of fungal growth and development. Nat Commun 11. 2020.  http://dx.doi.org/10.1038/s41467-020-17615-5.

Bonhomme, M., Bensmihen, S., André, O., Amblard, E., Garcia, M., Maillet, F., Puech-Pagès, V. et al. Distinct genetic bases for plant root responses to molecules from distinct microbial origins. bioRxiv 33: 285668. 2020. https://doi.org/10.1101/2020.09.09.285668.

Cope, K.R., Bascaules, A., Irving, T.B., Venkateshwaran, M., Maeda, J., Garcia, K., et al. The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots. Plant Cell 31: 2386–2410. 2019. https://doi.org/10.1105/tpc.18.00676.

Volpe, V., Carotenuto, G., Berzero, C., Cagnina, L., Puech-Pagès, V., and Genre, A. Short chain chito-oligosaccharides promote arbuscular mycorrhizal colonization in Medicago truncatula. Carbohydr Polym 229: 115505. 2019. https://doi.org/10.1016/j.carbpol.2019.115505

Gibelin-Viala, C., Amblard, E., Puech-Pagès, V., Bonhomme, M., Garcia, M., Bascaules-Bedin, A., et al. The Medicago truncatula LysM receptor-like kinase LYK9 plays a dual role in immunity and the arbuscular mycorrhizal symbiosis. New Phytol 223: 1516–1529, 2019. https://doi.org/10.1111/nph.15891.

Pellegrin, C., Daguerre, Y., Ruytinx, J., Guinet, F., Kemppainen, M., Frey, N.F. dit, Puech-Pagès, V., et al. Laccaria bicolor MiSSP8 is a small-secreted protein decisive for the establishment of the ectomycorrhizal symbiosis. Environ Microbiol 21: 3765–3779. 2019. https://doi.org/10.1111/1462-2920.14727