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Accueil du site > Equipes de recherche > Immunité Végétale et Effecteurs > Thèmes de recherche > Genetic determinism and mechanisms of resistance to Aphanomyce euteiches in the model legume Medicago truncatula

Genetic determinism and mechanisms of resistance to Aphanomyce euteiches in the model legume Medicago truncatula

Contact : mail to Christophe JACQUET, mail to Maxime BONHOMME

Due to a lack of efficient chemicals to control Aphanomyces euteiches (Ae) development, a pivotal research axis is the genetic improvement of crop legumes resistance and a better understanding of the mechanisms set up by the innate immune system in plants. To speed up this research, the model legume M. truncatula (Mt), host of Ae, has been selected to develop a pathosystem and to study quantitative resistance observed towards the oomycete.

Until now, analyses highlighted specific features of resistance :

-  Resistance is linked to different mechanisms (see figure) leading to the protection of the root stele against Ae.

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Comparison of observed phenotypes for partially resistant (top) and susceptible (bottom) lines of M. truncatula, 15 days after inoculation with A. euteiches.

-  Fine mapping localized the genomic region involved in this resistance on chromosome 3 in a less than 100 kb interval, and this region does not contain “classical” resistance genes, thus suggesting a mechanism still not outlined.

In the frame of the “Immunit-Ae” project funded by the French National Research Agency (ANR) and supervised by the team, the main objectives are i) to clone the gene(s) of the locus involved in the resistance to different Ae strains ii) to unravel molecular mechanisms activated by this/these gene(s) using a transcriptomic approach and iii) to use association mapping strategies which mine Mt natural diversity, in order to identify new genomic regions involved in resistance to Ae. Work on these tasks will be achieved with the collaboration of research teams located at INRA (Rennes, Montpellier ; France), Evry (France) and the University of Minnesota (USA).