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Accueil du site > Equipes de recherche > Signalisation calcique > Thèmes de recherche > Calcium sensors and environmental stresses

Calcium sensors and environmental stresses

Contacts : mail to Didier ALDON, mail to Jean-Philippe GALAUD, mail to Benoît RANTY, mail to Christian Mazars

Like all living organisms, plants are subjected to the constraints of their environment, of biotic (bacteria, fungi, viruses ...) or abiotic (drought, soil salinity, extreme temperatures ...) natureand have to cope with them to survive. Mechanisms that trigger specific responses to the nature and intensity of the perceived signal will depend on the ability of plants to perceive these environmental stimuli, to decode them and to activate the appropriate genetic programs. However, the molecular bases underlying mechanisms of perception, signaling and regulation of gene expression activated upon environmental constraints are still poorly understood. It is now well established that the plant ability to perceive these environmental changes lead to variations in calcium levels in the plant cells which are an essential element in the development of responses to environmental stresses . Among the proteins in charge of decoding and relaying these calcium signals, calmodulin (CaM) is a protein found in all eukaryotes and is able to interact and modulate the activity of various target proteins. Besides the typical form of CaM, plants have a particular directory of CaM-like proteins designated CML (calmodulin-like), whose physiological roles are unknown in most cases (Perochon et al., 2011, Zhu et al. 2015).

The work developed in the team using the model plant Arabidopsis provides new data on the physiological function of some CaMs and CMLs and of their targets in the tolerance to stress. Thus, we have shown that CML9 acts as a regulator in the responses to biotic (see figure) and abiotic stresses. Therefore our goals aims at studying the CML9 role and those of its recently identified targets in the establishment of plant responses to environmental stresses. Moreover, recent approaches engaged on other Arabidopsis CMLs indicate that CMLs participate in multiple functions. (Magnan et al., 2008, Perochon et al., 2010, Leba et al., 2012, Zhu et al. 2015).

Collaborations :

Hanin M. Sfax, Tunisie : http://www.cbs.rnrt.tn/

_Snedden W, Kingston, Canada. : http://post.queensu.ca/ sneddenw

_Mithöfer A., Iena, Allemagne : http://www.ice.mpg.de/ext/bioorgani...

_Berthomé R., Toulouse, France : http://www6.toulouse.inra.fr/lipm/R...

_Claire Lurin, Saclay, France : http://www.ips2.u-psud.fr

_Participation to « réseau résistance des plantes » structured by JB Morel & L Deslandes (Département SPE, INRA)

References

Ranty B, Aldon D, Cotelle V, Galaud JP, Thuleau P, Mazars C. (2016) Calcium sensors as key hubs in plant responses to biotic and abiotic stresses. Front. Plant Sci 7 : 327 doi : 10.3389/fps.2016.00327

Mazars C ; Ranty B ; Aldon D ; Oelmüller R ; Galaud J-P ; Mithöfer A (2016) CMLs control host-plant interactions. Journal of Endocytobiosis and Cell Research. 27, 13-19

Ghorbel M, Zaidi I, Robe E, Ranty B, Mazars C, Galaud JP, Hanin M. (2015) The activity of the wheat MAP kinase phosphatase 1 is regulated by manganese and by calmodulin. Biochimie. 108:13-9

Zhu X, Dunand C, Snedden W, Galaud JP. (2015) CaM and CML emergence in the green lineage. Trends Plant Sci. 8:483-9.

Cheval C, Aldon D, Galaud JP, Ranty B. (2013) Calcium/calmodulin-mediated regulation of plant immunity. Biochim Biophys Acta. 7:1766-71.

Thuleau P, Aldon D, Cotelle V, Brière C, Ranty B, Galaud JP, Mazars C. (2013) Relationships between calcium and sphingolipid-dependent signalling pathways during the early steps of plant-pathogen interactions. Biochim Biophys Acta. 7:1590-4.

Leba L-J, Cheval C, Ortiz-Martin I, Ranty B, Beuzon CR, Galaud J-P, Aldon C. (2012) CML9, an Arabidopsis calmodulin-like protein, contributes to plant innate immunity through a flagellin-dependent signaling pathway. Plant J. 71 : 976-989.

Leba LJ, Perochon A, Cheval C, Ranty B, Galaud JP, Aldon D.(2012). CML9, a multifunctional Arabidopsis thaliana calmodulin-like protein involved in stress responses and plant growth ? Plant Signal Behav. 7(9):1121-4.

Ranty B, Cotelle V, Galaud JP, Mazars C. (2012). Nuclear calcium signaling and its involvement in transcriptional regulation in plants. Adv Exp Med Biol. 740:1123-43.

Perochon A, Aldon D, Galaud J-P, Ranty B. (2011) Calmodulin and calmodulin-like proteins in plant calcium signaling. Biochimie 93 : 2048-2053.

Perochon A, Dieterle S, Pouzet C., Aldon D, Galaud J-P, Ranty B. (2010) Interaction of a plant pseudo-response regulator with a calmodulin-like protein. B.B.R.C. 398 : 747-751.

Magnan F, Ranty B, Charpenteau M, Sotta B, Galaud JP, Aldon D. (2008) Mutations in AtCML 9, a calmodulin−like protein from Arabidopsis thaliana, alter plant responses to abiotic stress and abscisic acid. Plant J. 56 : 575−589.